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Objective: To investigate the reliability and validity of a newly developed semistructured interview, the Algorithmically Structured Systematic Exploration of Subject's State of Mind (Assess_Mind). Method: Twelve scales have been developed and used to rate 86 Assess_Mind interviews conducted with patients undergoing in vitro fertilization treatment. For each rating scale, we assessed interrater reliability, interviewer effect, and construct validity. In addition, a factor analysis of scales was performed. To study the validity of scales and of the factors yielded by factor analysis, patients were assessed with a psychopathological battery including the Child Project Questionnaire, the State-Trait Anxiety Inventory, the Perceived Stress Scale, and the Ways of Coping Checklist. Results: For 11 of the 12 scales, interrater reliability was fair to excellent. There was no significant interviewer effect affecting any of the scales. In addition, the validity of nine of these 11 scales was demonstrated by their correlations with questionnaires measuring similar constructs. Conclusion: Nine of the Assess_Mind scales appear to have adequate psychometric properties.