Following recent debates within Psychosocial Studies, this paper explores the interpretive trajectories initiated in contrasting conceptualisations of the relation between subject and other. Starting from a discussion of countertransference, I go on to examine Lacan’s notion of the ‘action of interpretation’ and what this might look like within the practice of research. My analysis is organised around instances from an interview-based research project investigating unconscious relations in academic practice. These instances relate to moments of disruption to disciplinary or methodological identities. The analysis thus draws attention to shifting locations and modes of articulation of desire within research.