The analyst's self-analysis—originally fashioned on Freud's solo foray into his own unconscious mind—continues to play an important psychoanalytic role. A summary of relevant literature is presented that includes recent relational psychoanalytic and neuroscientific data. Three major findings emerge: First, analysts' achievement of self-awareness in the analytic setting is clearly limited, more limited than we might like to admit, especially when we act alone; second, analysts reaching clinical self-awareness is a mutual, interactive process that, in addition to psychological processes, can be understood on the basis of operations uncovered by neuroscience, especially the mirror neuron system; third, accordingly, a form of “mutual” analysis is seen as an indispensable element of the analytic process. Analysts' achievement of self-awareness is discussed with a particular focus on our intrinsic relationality, and on mentalization, self-reflexivity, new relational experience, and therapeutic action. Illustrative case material is discussed.