Following the idea of Green about whether sexuality is related to psychoanalysis, I begin this article with a question that refers to the title of the work: “Has castration anxiety anything to do with psychoanalysis?” Concerned about the lack of bibliographic reference to the concept of castration anxiety, I make a tour of the notion, taking as its point of departure and arrival the intellectual itinerary of Freud. I continue with a brief review of later authors. Interested in rethinking this issue, I wonder whether castration anxiety is expressed in the same manner in the clinical practice of today. The question does not tolerate a hasty response, so I present two clinical vignettes to call attention to castration anxiety as a central factor in the development and understanding of the clinical practice. The last section refers to the discussion and concluding reflections.